Resource Categories: Publications Resources

Publication: A novel hydrogen peroxide evolved CHO host can improve the expression of difficult to express bispecific antibodies

Publication featuring ChemStress®. The manufacture of bispecific antibodies by Chinese hamster ovary (CHO) cells is often hindered by lower product yields compared to monoclonal antibodies. Publication outlines the generation of a novel hydrogen peroxide evolved host using directed host cell evolution.

Mistry et al., Biotechnology and Bioengineering, 118, (2021) 2326–2337.

Active cell nucleus splitting

Publication: Cell function profiling to assess clone stability

Publication featuring ChemStress®. In cell line development the identification of stable CHO cells for production is a critical but onerous task. This publication outlines the use of ChemStress® cell function profiling that uses a panel of active chemicals to mimic known bioprocess stresses and challenge key pathways.

Dobson et al., Biotechnology and Bioengineering. 2020; 1–5

Biological cells

Publication: A platform for context-specific genetic engineering of recombinant protein production by CHO cells

Publication describes and validates the development of a high-throughput microscale platform featuring Valita®Titer technology.  The platform was used to compare and identify optimal cell engineering solutions for both transient and stable production of a model DTE IgG1 monoclonal antibody.

Cartwright et al., Journal of Biotechnology 312 (2020) 11–22


Publication: High-throughput quantitation of Fc-containing recombinant proteins in cell culture supernatant by fluorescence polarization spectroscop

Publication featuring Valita®Titer. Measurement of recombinant protein product titer critically underpins all biopharmaceutical manufacturing process development. This publication describes the use of Valita®Titer, a rapid 96-well assay, that enables high-throughput quantitation of recombinant immunoglobulin G and Fc-containing IgG derivatives in mammalian cell culture supernatant. 

Thompson et al., Analytical Biochemistry, 534 (2017) 49-55