Resource Categories: Publications Resources

Publication: A novel hydrogen peroxide evolved CHO host can improve the expression of difficult to express bispecific antibodies

Publication featuring ChemStress. The manufacture of bispecific antibodies by Chinese hamster ovary (CHO) cells is often hindered by lower product yields compared to monoclonal antibodies. Publication outlines the generation of a novel hydrogen peroxide evolved host using directed host cell evolution.

Mistry et al., Biotechnology and Bioengineering, 118, (2021) 2326–2337.

Active cell nucleus splitting

Publication: Cell function profiling to assess clone stability

Publication featuring ChemStress. In cell line development the identification of stable CHO cells for production is a critical but onerous task. This publication outlines the use of ChemStress cell function profiling that uses a panel of active chemicals to mimic known bioprocess stresses and challenge key pathways.

Dobson et al., Biotechnology and Bioengineering. 2020; 1–5

Biological cells

Publication: A platform for context-specific genetic engineering of recombinant protein production by CHO cells

Publication describes and validates the development of a high-throughput microscale platform featuring ValitaTiter technology.  The platform was used to compare and identify optimal cell engineering solutions for both transient and stable production of a model DTE IgG1 monoclonal antibody.

Cartwright et al., Journal of Biotechnology 312 (2020) 11–22


Publication: High-throughput quantitation of Fc-containing recombinant proteins in cell culture supernatant by fluorescence polarization spectroscop

Publication featuring ValitaTiter. Measurement of recombinant protein product titer critically underpins all biopharmaceutical manufacturing process development. This publication describes the use of ValitaTiter, a rapid 96-well assay, that enables high-throughput quantitation of recombinant immunoglobulin G and Fc-containing IgG derivatives in mammalian cell culture supernatant. 

Thompson et al., Analytical Biochemistry, 534 (2017) 49-55